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Original R esearch Article
Susceptibility Pattern of a Clinical Isolate of
Staphylococcus aureus to the Combined Activity of a
Herbal Preparation of Azadirachta indica and Some
Antibiotics
Ngwu MI1,2, Adikwu MU1,
Ibezim EC1,
Odimegwu DC1*, Ngwu
GI3 , Esimone CO1
1Division
of Pharmaceutical Microbiology, Department of
Pharmaceutics, University of Nigeria, Nsukka, Enugu
State, Nigeria
2Department
of Veterinary Microbiology and Pathology, University of
Nigeria, Nsukka, Enugu State, Nigeria.
3Department
of Zoology, University of Nigeria, Nsukka, Enugu State,
Nigeria.
2Department
of Zoology, University of Benin, Benin City, Edo State,
Nigeria.
*For correspondence: Tel:
+234-803-709-4427 Email:
nonsodimegwu@yahoo.co.uk
Received:
30-Apr-09 Revised:
18-May-09 Accepted:
03-Aug-09
International Journal of Health Research,
September
2009; 2(3):
279-286
Abstract
Purpose:
To investigated the in vitro antimicrobial
interaction between the crude water extract of
Azadirachta indica and some standard antibiotics;
doxycycline, gentamicin, streptomycin, erythromycin,
ciprofloxacin, and norfloxacin.
Methods:
The in vitro interactions were evaluated using a
combination of agar diffusion and Checkerboard
techniques against Staphylococcus aureus as test
microorganism.
Results:
There were synergistic interactions between the crude
extract (0.1 mg/ml or 0.25mg/ml extract) and all the
antibiotics used except tetracycline in the agar
diffusion technique (p< 0.05). In the Checkerboard
technique, fractional inhibitory concentration (FIC)
index reveal that the crude extract were synergistic
with either tetracycline (1:9), streptomycin (1:9, 9:1),
ciprofloxacin (2:8, 5:5) or norfloxacin (2:8, 6:4).
Conclusion:
There is a possible beneficial clinical application of
the co-administration of doxycycline, gentamicin,
streptomycin, erythromycin, ciproflo-xacin or
norfloxacin and the crude extract of A. indica in
the treatment of infections caused by S. aureus.
However, unguided concomitant usage may result in
therapeutic failure.
Keywords:
Staphylococcus
aureus,
interaction, antibiotics, FIC index |
